The causative agent of Syphilis is a bacterium: Treponema pallidum. The immune responses to T. pallidum are primarily mediated by the following membrane lipoproteins: TpN47, TpN17, TpN15 and TmpA. TpN47 is highly immunogenic and activates endothelial cells, while TpN17 and TpN15 induce antibody responses.
In independent studies, TmpA is the most frequently identified antigen (95%) while the remaining are recognized with the following prevalence: TpN47, 92.5%; TpN17, 89.5%; and TpN15, 67.5%.
Recombinant protein corresponding to Syphilis TpN47 expressed in E. coli.
Molecular Weight:~47.7kD
Activity:Binds Syphilis antibodies
Applications:Suitable for use in ELISA, Western Blot and Lateral Flow. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：1mg
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：≥95% (SDS-PAGE)
Form：Supplied as a lyophilized powder in 20mM carbonate, pH 10.0. No preservative added.
