Typhoid fever is an acute systemic illness caused by Salmonella enterica serovar Typhi. Typhoid fever remains a public health concern, with an estimated 22 million cases and 200,000 related deaths occurring worldwide each year.
The S. Typhi HlyE protein belongs to a class of pore-forming toxins with hemolytic and cytotoxic activities that are absent in other Salmonella enterica serovars. The HlyE protein is expressed as a monomer, but it has a tendency to form non-functional soluble multimers in aqueous solution. Serological studies have shown that S. Typhi and S. Paratyphi A strains produce substantial amounts of HlyE during human infection.
S. Typhi HlyE has been found in the plasma of patients with S. Typhi bacteremia and recent studies have identified that HlyE is an antigen that is capable of discriminating between typhoid patients and control subjects. The reactivity of different Ig isotypes (IgG, IgA and IgM) against rHlyE has also been demonstrated by ELISA. (Ong et al. Microbial Immunol 2015; 59:43-47).
Recombinant protein corresponding to Typhoid HylE expressed in E. coli.
Molecular Weight:~34kD
Activity:Binds typhoid positive serum antibodies
Applications:Suitable for use in ELISA, Western Blot and Lateral Flow. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：500ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：≥95% (SDS-PAGE)
Form：Supplied as a lyophilized powder in 20mM Na-carbonate, pH 9.6. No preservative added.
