HIV-2 is a phylogenetic relative of HIV-1, which is reported to be less infectious and induce a slower progression to AIDS than HIV-1. Accordingly, the clinical outcomes and treatment approaches for HIV-1 and HIV-2 infection are different, and distinguishing between the two strains is an important diagnostic objective. Despite extensive sequence heterology between HIV-1 and HIV-2, the structures of the HIV-1 and HIV- 2 envelope proteins are similar, with HIV-2 gp36 mediating the fusogenic activity of the HIV-2 envelope, and representing the structural and functional equivalent of HIV-1 gp41. The immunodominant regions of gp41 and gp36 are, however, sufficiently distinct such that antibodies generated against HIV- 1 and HIV-2 do not cross-react significantly with their respective cognate gp41 and gp36 targets. As such recombinant gp36 is commonly used in diagnostic immunoassays to distinguish between HIV-1 and HIV-2 infections.
Source:Recombinant protein corresponding to HIV-2 gp36 expressed in E. coli.
Molecular Weight:~17kD
Activity:Binds HIV-2 positive serum antibodies
Applications:Suitable for use in ELISA, Western Blot and Lateral Flow. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：1mg
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：≥95% (SDS-PAGE)
Form：Supplied as a liquid in 50mM Na-formate, pH 3.0. No preservative added.
