85-4625-69　［取扱停止］Tumor Necrosis Factor-alpha, Recombinant, Human, His-Tag, aa77-233 50ug　361386

特徴

• Source:Recombinant protein corresponding to human Tumor Necrosis Factor-alpha, a single, non-glycosylated, polypeptide chain containing 157aa fragment (aa77-233), fused to His-Tag at N-terminal, expressed in E. coli.
• Molecular Weight:~17.5kD (157aa)
• Applications:Suitable for use in SDS-PAGE. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Biological Activity:Fully biologically active when compared to standard. The specific activity is >2×10e7IU/mg as determined by the cytolysis of mouse L929 cells in the presence of Actinomycin D.
• Endotoxin:<1EU/mg (LAL)
• AA Sequence:MHHHHHHVRS SSRTPSDKPV AHVVANPQAE GQLQWLNRRA NALLANGVEL RDNQLVVPSE GLYLIYSQVL FKGQGCPSTH VLLTHTISRI AVSYQTKVNL LSAIKSPCQR ETPEGAEAKP WYEPIYLGGV FQLEKGDRLS AEINRPDYLD FAESGQVYFG IIAL
• Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with 10mM HAc. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.