85-4625-67　［取扱停止］Tumor Necrosis Factor-alpha (TNF-alpha), Recombinant, Human 50ug　361385

特徴

• Source:Recombinant protein corresponding to a mixture of a 158aa residue form containing an N-terminal methionine and a 157aa residue form with the sequence of mature human Tumor Necrosis Factor-alpha (rHuTNF-alpha) expressed in E. coli.
• Molecular Weight:~17.5kD (158aa)
• Applications:Suitable for use in SDS-PAGE. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Biological Activity:Fully biologically active when compared to standard. The specific activity is >2×107IU/mg as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D.
• Endotoxin:<1EU/mg (LAL)
• AA Sequence:VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVVPSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL
• Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile dH2O, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.