85-4625-64　［取扱停止］Tumor Necrosis Factor alpha, Recombinant, Mouse (TNF-alpha) 5ug　361383

特徴

• Tumor Necrosis Factor-alpha (TNF-alpha) is a potent lymphoid factor which exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.
• Source:Recombinant protein corresponding to mouse Tumor Necrosis Factor-alpha, a soluble 157aa protein which corresponds to C-terminal extracellular domain of the full length transmembrane protein, expressed in E. coli.
• Molecular Weight:~17.3kD (157aa)
• Applications:Suitable for use in SDS-PAGE. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Biological Activity:The ED50 as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is <0.1ng/ml, corresponding to a specific activity of > 1×10e7units/mg.
• Endotoxin:<1EU/mg (LAL)
• AA Sequence:MLRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEKVNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL
• Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile dH2O, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.