85-4625-27　［取扱停止］Sonic HedgeHog, Recombinant, Human (Shh) 25ug　361220

特徴

• Sonic hedgehog (SHH) is a member of a small group of secreted proteins that are essential for development in both vertebrates and invertebrates. Three mammalian hedgehog genes (sonic, desert, Indian) share about 60% homology and all signal via the same receptors.
• Source:Recombinant protein corresponding to human Sonic HedgeHog, a single non-glycosylated polypeptide chain containing 176aa, expressed in E. coli.
• Molecular Weight:~20kD (176aa)
• Applications:Suitable for use in SDS-PAGE. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Biological Activity:Determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) cells. The expected ED50 for this effect is 0.8-1ug/ml, corresponding to a specific activity of ＞1x10e3IU/mg.
• Endotoxin:<1EU/ug (LAL)
• AA Sequence:IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRALDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG
• Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile dH2O, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.