Intercellular adhesion molecule 1 (ICAM-1), also known as CD54, binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS. Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface.
Sample Type:Cell culture supernatants, serum, plasma, and tissue
Intended Use:The Human sICAM-1 BioAssay™ ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Human sICAM-1 concentrations in cell culture supernatants, serum, plasma, and tissue.
Sensitivity:60pg/ml
Range:125-8000pg/ml
Specificity:Recognizes human sICAM-1.
Sample Volume:100ul/well
Test Principle:This assay employs the Sandwich immunoassay technique. An anti-h sICAM-1 monoclonal coating antibody is adsorbed onto microwells. sICAM-1 present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated anti-h sICAM-1 antibody is added and binds to sICAM-1 captured by the first antibody. Following incubation unbound Biotinylated anti-h sICAM-1 antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-h sICAM-1 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of sICAM-1 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven sICAM-1 standard dilutions and sICAM-1 sample concentration determined.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
