Resistin, also known as adipocyte-specific secretory factor (ADSF) and found in inflammatory zone 3 (FIZZ3), is an adipocyte-derived protein. Mature mouse and human Resistin share 56%aa sequence identity. Northern blot analysis shows that Resistin is expressed in mouse adipose tissue, and its expression is markedly induced during adipocyte differentiation of 3T3-L1 preadipocyte cells.
Sample Type:Cell culture supernatants, serum, plasma, and tissue
Intended Use:The Human Resistin BioAssay™ ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Human Resistin concentrations in cell culture supernatants, serum, plasma, and tissue.
Sensitivity:70pg/ml
Range:156-10000pg/ml
Specificity:Recognizes human Resistin.
Sample Volume:100ul/well
Test Principle:This assay employs the Sandwich immunoassay technique. An anti-h Resistin monoclonal coating antibody is adsorbed onto microwells. Resistin present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated anti-h Resistin antibody is added and binds to Resistin captured by the first antibody. Following incubation unbound Biotinylated anti-h Resistin antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-h Resistin antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of Resistin present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven Resistin standard dilutions and Resistin sample concentration determined.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
