The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).
Sample Type:Cell culture supernatants, serum, plasma, and tissue
Intended Use:The Human MMP-2 BioAssay™ ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Human MMP-2 concentrations in cell culture supernatants, serum, plasma, and tissue.
Specificity:Recognizes human MMP-2.
Sample Volume:100ul/well
Test Principle:This assay employs the Sandwich immunoassay technique. An anti-h IL-18 monoclonal coating antibody is adsorbed onto microwells. IL-18 present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated anti-h IL-18 antibody is added and binds to IL-18 captured by the first antibody. Following incubation unbound Biotinylated anti-h IL-18 antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-h IL-18 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of IL-18 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-18 standard dilutions and IL-18 sample concentration determined.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
