85-4624-62　［取扱停止］MIP-1 alpha, Recombinant, Human (Macrophage Inflammatory Protein 1 alpha, CCL3) 20ug　360784

特徴

• Macrophage Inflammatory Proteins (MIP) belong to the family of chemotactic cytokines known as chemokines. In humans, there are two major forms, MIP- 1a and MIP-1b that are now officially named CCL3 and CCL4 respectively. Both are major factors produced by macrophages after they are stimulated with bacterial endotoxins. They activate human granulocytes (neutrophils, eosinophils and basophils) which can lead to acute neutrophilic inflammation. They also induce the synthesis and release of other pro-inflammatory cytokines such as interleukin 1(IL-1), IL-6 and TNF-alphafrom fibroblasts and macrophages. The genes for CCL3 and CCL4 are both located on human chromosome 17.
• Source:Recombinant protein corresponding to human MIP-1 alpha, containing 69aa residues, including the four highly conserved cysteine residues present in CC chemokines, expressed in E. coli.
• Molecular Weight:~7.8kD (69aa)
• Applications:Suitable for use in SDS-PAGE. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Biological Activity:Determined by its ability to chemoattract human monocytes using a concentration range of 1-10ng/ml, corresponding to a specific activity of ＞1x10e5IU/mg.
• Endotoxin:<1EU/mg (LAL)
• AA Sequence:ASLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPGV IFLTKRSRQV CADPSEEWVQ KYVSDLELSA
• Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile dH2O, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.