MCP-1, also known as monocyte chemotactic and activating factor (MCAF), was initially purified based on its ability to chemoattract monocytes. Subsequent to its cloning and sequencing, it became evident that this protein is also identical to the product of the human JE gene. The JE gene, originally identified in mouse fibroblasts, is a platelet-derived growth factor (PDGF)-inducible gene.
Sample Type:Cell culture supernatants, serum, plasma, and tissue
Intended Use:The Mouse MCP-1 BioAssay™ ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Mouse MCP-1 concentrations in cell culture supernatants, serum, plasma, and tissue.
Sensitivity:2pg/ml
Range:250-3.9pg/ml
Specificity:Recognizes mouse MCP-1.
Sample Volume:100ul/well
Test Principle:This assay employs the Sandwich immunoassay technique. A MCP-1 monoclonal coating antibody is adsorbed onto microwells. MCP-1 present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated anti-m MCP-1 antibody is added and binds to MCP-1 captured by the first antibody. Following incubation unbound Biotinylated anti-m MCP-1 antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-m MCP-1 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of MCP-1 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven MCP-1 standard dilutions and MCP-1 sample concentration determined.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
