85-4624-33　［取扱停止］MCAF, Recombinant, Human (Monocyte Chemotactic Protein-1, MCP-1, CCL2, Monocyte Chemotactic and Activating Factor) 5ug　360743

特徴

• Chemokine (C-C motif) ligand 2 (CCL2) is a small cytokine belonging to the CC chemokine family that is also known as monocyte chemotactic protein-1 (MCP-1). It is found at the site of tooth eruption and bone degradation. In the bone, CCL2 is expressed by mature osteoclasts and osteoblasts and is under the control of nuclear factor B (NFB). CCL2 recruits immune cells, such as monocytes, to sites of tissue injury and infection. This chemokine is produced as a protein precursor containing signal peptide of 23aa and a mature peptide of 76aa. It is a monomeric polypeptide, with a molecular weight of ~13kD. As with many other CC chemokines, CCL2 is located on chromosome 17 in humans. The cell surface receptors that bind CCL2 are CCR2 and CCR5.
• Source:Recombinant protein corresponding to human MCP-1/MCAF, a single non-glycosylated polypeptide chain containing 76aa, expressed in E. coli.
• Molecular Weight:~8.6kD (76aa)
• Applications:Suitable for use in SDS-PAGE. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Biological Activity:Measured by its ability to chemoattract human CCR2A transfected BaF3 mouse pro-B cells. The ED50 for this effect is typically 10-60ng/mL, corresponding to a specific activity of ＞1.7x10e4IU/mg.
• Endotoxin:<1EU/mg (LAL)
• AA Sequence:QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT
• Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile dH2O, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.