85-4624-10　［取扱停止］Kallikrein-1, Recombinant, Human, His-Tag (KLK-1) 100ug　360634

特徴

• Source:Recombinant protein corresponding to human Kallikrein-1, a single non-glycosylated polypeptide chain containing 262aa with 6×His-Tag at C-terminal, expressed in E. coli.
• Molecular Weight:~29.7kD (262aa)
• Applications:Suitable for use in SDS-PAGE. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Biological ActivityMeasured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4- methylcoumarin (PFRAMC). The specific activity is > 2000pmoles/min/ug.
• Endotoxin:<1EU/mg (LAL)
• AA Sequence:MWFLVLCLAL SLGGTGAAPP IQSRIVGGWE CEQHSQPWQA ALYHFSTFQC GGILVHRQWV LTAAHCISDN YQLWLGRHNL FDDENTAQFV HVSESFPHPG FNMSLLENHT RQADEDYSHD LMLLRLTEPA DTITDAVKVV ELPTEEPEVG STCLASGWGS IEPENFSFPD DLQCVDLKIL PNDECKKAHV QKVTDFMLCV GHLEGGKDTC VGDSGGPLMC DGVLQGVTSW GYVPCGTPNK PSVAVRVLSY VKWIEDTIAE NSHHHHHH
• Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile dH2O, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.