85-4623-73　［取扱停止］Interleukin-13, Variant, Recombinant, Human (IL-13 Variant) 10ug　360592

特徴

• IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and also by mast cells and NK cells. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 exerts anti-inflammatory effects on monocytes and macrophages and it inhibits the expression of inflammatory cytokines such as IL-1b, TNF-a, IL-6 and IL-8. IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching resulting in increased production of IgE. Blocking of IL-13 activity inhibits the pathophysiology of asthma. Human and mouse IL-13 is cross-species reactive. A variant of IL-13 shows enhanced functional activity compared with the wild type IL-13.
• Source:Recombinant protein corresponding to human Interleukin-13 Variant, a single non-glycosylated polypeptide chain containing 114aa, with a substitution of Q for R at position 112 compared with the wild type IL-13, expressed in E. coli.
• Molecular Weight:~12.5kD (114aa)
• Applications:Suitable for use in SDS-PAGE. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Biological ActivityFully biologically active when compared to standard. The ED50 as determined by the dose-dependent proliferation of TF-1 cells was <1ng/ml, corresponding to a specific activity of >1×10e6units/mg. This analog has also been shown to exhibit increased in vivo activity compared to wild type IL-13.
• Endotoxin:<1EU/mg (LAL)
• AA Sequence:SPGPVPPSTA LRELIEELVN ITQNQKAPLC NGSMVWSINL TAGMYCAALE SLINVSGCSA IEKTQRMLSG FCPHKVSAGQ FSSLHVRDTK IEVAQFVKDL LLHLKKLFRE GQFN
• Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile dH2O, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.