85-4623-63　［取扱停止］Interleukin-10, Recombinant, Mouse (IL-10) 10ug　360586

特徴

• Source:Recombinant protein corresponding to mouse Interleukin-10, a single non-glycosylated polypeptide chain containing 161aa, expressed in E. coli.
• Molecular Weight:~18.7kD (161aa)
• Applications:Suitable for use in SDS-PAGE. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Biological Activity:Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent co-stimulation (with IL-4) of the proliferation of mouse MC/9 cells was found to be <2ng/ml, corresponding to a specific activity of >5x10e5units/mg.
• Endotoxin:<1EU/mg (LAL)
• AA Sequence:MSRGQYSRED NNCTHFPVGQ SHMLLELRTA FSQVKTFFQT KDQLDNILLT DSLMQDFKGY LGCQALSEMI QFYLVEVMPQ AEKHGPEIKE HLNSLGQKLK TLRMRLRRCH RFLPCENKSK AVEQVKSDFN KLEDQGVYKA MNEFDIFINC IEAYMMIKMK S
• Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile dH2O, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.