85-4623-28　［取扱停止］Insulin-Like Growth Factor I, Recombinant, Rat (IGF-1) 10ug　360567

特徴

• IGF-1 (insulin-like Growth Factor-1) is a hormone similar in molecular structure to insulin. It is a polypeptide growth factor that stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. Insulin-like growth factor 1 has been shown to bind and interact with all the IGF-1 Binding Proteins (IGFBPs), of which there are six (IGFBP1-6). IGFBP-3, the most abundant protein, accounts for 80% of all IGF binding. IGF-1 binds to IGFBP-3 in a 1:1 molar ratio. IGF-1 binds to at least two cell surface receptors: the IGF-1 receptor (IGF1R), and the insulin receptor. The IGF-1 receptor seems to be the "physiologic" receptor - it binds IGF-1 at significantly higher affinity than the IGF-1 that is bound to the insulin receptor.
• Source:Recombinant protein corresponding to human Insulin-like Growth Factor-1, a single non-glycosylated polypeptide chain containing 70aa, expressed in E. coli.
• Molecular Weight:~7.69kD (70aa)
• Applications:Suitable for use in SDS-PAGE. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Biological Activity:The ED50 as determined by a cell proliferation assay using FDC-P1 cells is <2.0ng/ml, corresponding to a specific activity of >5×10e5units/mg.
• Endotoxin:<1EU/mg (LAL)
• AA Sequence:GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKSA
• Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile dH2O, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.