CXCL8 was originally discovered and purified as a neutrophil chemotactic and activating factor. It was also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocytederived neutrophil chemotactic factor (MDNCF), T lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce CXCL8 in response to a wide variety of proinflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. CXCL8 is a member of the alpha (CXC) subfamily of chemokines, which also includes platelet factor-4, GRO, and IP10.
Sample Type:Cell culture supernatants, serum, plasma, and tissue
Intended Use:The Human IL-8 BioAssay™ ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Human IL-8 concentrations in cell culture supernatants, serum, plasma, and tissue.
Sensitivity:2pg/ml
Range:7.8-500pg/ml
Specificity:Recognizes human IL-8.
Sample Volume:100ul/well
Test Principle:This assay employs the Sandwich immunoassay technique. An anti-h IL-8 monoclonal coating antibody is adsorbed onto microwells. IL-8 present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated anti-h IL-8 antibody is added and binds to IL-8 captured by the first antibody. Following incubation unbound Biotinylated anti-h IL-8 antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-h IL-8 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of IL-8 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-8 standard dilutions and IL-8 sample concentration determined.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
