Interleukin 5 (IL-5) is a T cell-derived factor that promotes the proliferation, differentiation and activation of eosinophils. In mice, IL-5 is also a growth and differentiation factor for B cells. Various names previously used to describe IL-5 include: Tcell replacing factor (TRF), B cell growth factor II (BCGFII), B cell differentiation factor u (BCDF u), eosinophil differentiation factor (EDF) and eosinophil colony-stimulating factor (Eo-CSF). Biologically active IL-5 is a disulfide-linked homodimer. Rat IL-5 is 94% and 70% identical to mouse and human IL-5, respectively.
Sample Type:Cell culture supernatants, serum, plasma, and tissue
Intended Use:The Human IL-5 BioAssay™ ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Human IL-5 concentrations in cell culture supernatants, serum, plasma, and tissue.
Sensitivity:2pg/ml
Range:3.9-250pg/ml
Specificity:Recognizes human IL-5.
Sample Volume:100ul/well
Test Principle:This assay employs the Sandwich immunoassay technique. An anti-h IL-5 monoclonal coating antibody is adsorbed onto microwells. IL-5 present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated anti-h IL-5 antibody is added and binds to IL-5 captured by the first antibody. Following incubation unbound Biotinylated anti-h IL-5 antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-h IL-5 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of IL-5 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-5 standard dilutions and IL-5 sample concentration determined.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
