Interleukin 4 (IL-4) is a pleiotropic cytokine produced primarily by activated T lymphocytes, mast cells and basophils. IL-4 has multiple immune response-modulating activities on a variety of cell types. It is an important regulator of isotype switching, inducing IgE production in B lymphocytes. It is an important modulator of the differentiation of precursor T helper cells to the Th2 subset that mediates humoral immunity and modulates antibody production. In addition, IL-4 has also been shown to have anti-tumor activity both in vivo and in vitro. The cDNA sequence of mouse IL-4 predicts a 140aa residue precursor protein containing a 20aa residue signal peptide that is cleaved to form the mature protein. At the amino acid sequence level, mature mouse IL-4 is approximately 50% identical to human IL-4 but there is no species cross-reactivity for biological activity for the two proteins.
Sample Type:Cell culture supernatants, serum, plasma, and tissue
Intended Use:The Mouse IL-4 BioAssay™ ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Mouse IL-4 concentrations in cell culture supernatants, serum, plasma, and tissue.
Sensitivity:7pg/ml
Range:15.625-1000pg/ml
Specificity:Recognizes mouse IL-4. There was no detectable cross reactivity with any of the tested proteins following IL-1,2,3,5,6,10, 8,12,TNF,VEGF,TGF and IFN.
Sample Volume:100ul/well
Test Principle:This assay employs the Sandwich immunoassay technique. An anti-m IL-4 monoclonal coating antibody is adsorbed onto microwells. IL-4 present in the sample or standard and Biotinylated anti IL-4 antibody binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard and Biotinylated anti IL-4 antibody are removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti IL-4 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of IL-4 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-4 standard dilutions and IL-4 sample concentration determined.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
