Interleukin 2 (IL-2) was initially identified as a T cell growth factor that is produced by T cells following activation by mitogens or antigens. Since then, it has also been shown to stimulate the growth and differentiation of B cells, natural killer (NK) cells, lymphocyte activated killer (LAK) cells, monocytes/macrophages and oligodendrocytes. At the amino acid sequence level, there is approximately 60% - 90% similarity between species. Mature human IL-2 shows 65%, 67%, 72%, 78%, and 64%aa identity to mouse, rat, porcine, feline, and bovine IL-2, respectively.
Sample Type:Cell culture supernatants, serum, plasma, and tissue
Intended Use:The Rat IL-2 BioAssay™ ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Rat IL-2 concentrations in cell culture supernatants, serum, plasma, and tissue.
Sensitivity:15pg/ml
Range:15.625-1000pg/ml
Specificity:Recognizes rat IL-2.
Sample Volume:100ul/well
Test Principle:This assay employs the Sandwich immunoassay technique. An anti-r IL-2 monoclonal coating antibody is adsorbed onto microwells. IL-2 present in the sample or standard and Biotinylated anti-IL-2 antibody binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard and Biotinylated anti-IL-2 antibody are removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-IL-2 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of IL-2 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-2 standard dilutions and IL-2 sample concentration determined.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
