Interleukin 1 (IL-1) includes two distinct proteins, IL-1alpha and IL-1beta, that play central roles in acute and chronic inflammation, both locally and systemically. Human IL-1beta is synthesized as a 269aa, 3kD precursor protein (prepro-IL-1beta) that is cleaved by IL-1beta-converting enzyme (ICE) to the 153aa, 17kD mature IL-1beta plus a prosegment. Some combination of the mature form, the prosegment and prepro-IL-1beta is released from the cell. IL-1beta is produced primarily by monocytes and macrophages but also by astrocytes, oligodendroglia, adrenal cortical cells, NK cells, endothelial cells, keratinocytes, megakaryocytes, platelets, neurons, neutrophils, osteoblasts, Schwann cells, trophoblasts, T cells, and fibroblasts. The most extensively studied function of IL-1 is initiation of inflammation. Bacterial endotoxin or a variety of non-microbial inflammatory substances induce production of IL-1, which is released into the local environment. IL-1 induces capillary endothelial cells to secrete chemokines (e.g. MCP-1) and to increase expression of cell adhesion molecules (e.g. E-selectin, ICAM-1 and VCAM-1).
Sample Type:Cell culture supernatants, serum, plasma, and tissue
Intended Use:The Human IL-1beta BioAssay™ ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Human IL-1beta concentrations in cell culture supernatants, serum, plasma, and tissue.
Sensitivity:4pg/ml
Range:7.8-500pg/ml
Specificity:Recognizes human IL-1beta. There was no detectable cross reactivity with any of the tested proteins following IL-2,3,4,5,6, 8,10,12,TNF,VEGF,TGF and IFN.
Sample Volume:100ul/well
Test Principle:This assay employs the Sandwich immunoassay technique. An anti-h IL-1beta monoclonal coating antibody is adsorbed onto microwells. IL-1beta present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated anti-h IL-1beta antibody is added and binds to IL-1beta captured by the first antibody. Following incubation unbound Biotinylated anti-h IL-1beta antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-h IL-1beta antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of IL-1beta present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-1beta standard dilutions and IL-1beta sample concentration determined.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
