Interleukin 11 (IL-11), also known as adipogenesis inhibitory factor (AGIF), has multiple effects on both hematopoietic and nonhematopoietic cells. Many of the biological effects described for IL-11 overlap those for IL-6. IL-11 exerts its biological activities through binding to a specific high-affinity receptor complex consisting of an IL-11 receptor chain and gp130.
Sample Type:Cell culture supernatants, serum, plasma, and tissue
Intended Use:The Mouse IL-11 BioAssay™ ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Mouse IL-11 concentrations in cell culture supernatants, serum, plasma, and tissue.
Sensitivity:15pg/ml
Range:31.25-2000pg/ml
Specificity:Recognizes mouse IL-11.
Sample Volume:100ul/well
Test Principle:This assay employs the Sandwich immunoassay technique. An anti-m IL-11 monoclonal coating antibody is adsorbed onto microwells. IL-11 present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated anti-m IL-11 antibody is added and binds to IL-11 captured by the first antibody. Following incubation unbound Biotinylated anti-m IL-11 antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-m IL-11 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of IL-11 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-11 standard dilutions and IL-11 sample concentration determined.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
