The Fibroblast Growth Factors (FGFs) constitute a large family of proteins involved in many aspects of development including cell proliferation, growth, and differentiation. They act on several cell types to regulate diverse physiologic functions including angiogenesis, cell growth, pattern formation, embryonic development, metabolic regulation, cell migration, neurotrophic effects, and tissue repair. FGF family activities are mediated by receptor tyrosine kinases and are facilitated by heparan sulfate. Family members have been implicated in several disorders of bone growth, as well as in tumor formation and progression.
Sample Type:Cell culture supernatants, serum, plasma, and tissue
Intended Use:The Human FGF BioAssay™ ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Human FGF concentrations in cell culture supernatants, serum, plasma, and tissue.
Sensitivity:7pg/ml
Range:15.625-1000pg/ml
Specificity:Recognizes human FGF.
Sample Volume:100ul/well
Test Principle:This assay employs the Sandwich immunoassay technique. An anti-h FGF basic monoclonal coating antibody is adsorbed onto microwells. FGF basic present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated anti-h FGF basic antibody is added and binds to FGF basic captured by the first antibody. Following incubation unbound Biotinylated anti-h FGF basic antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-h FGF basic antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of FGF basic present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven FGF basic standard dilutions and FGF basic sample concentration determined.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
