85-4621-61　［取扱停止］Epidermal Growth Factor, Recombinant, Mouse (EGF) 500ug　360189

特徴

• Epidermal Growth Factor is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53aa epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.
• Source:Recombinant protein corresponding to mouse Epidermal Growth Factor (rMuEGF) expressed in E. coli.
• Molecular Weight:~6kD (53aa)
• Applications:Suitable for use in SDS-PAGE. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Biological Activity:The ED50 as calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is <0.1ng/ml, corresponding to a specific activity of >1x10e7units/mg.
• Endotoxin:<1EU/mg (LAL)
• AA Sequence:NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR
• Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile dH2O, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.