85-4615-74　［取扱停止］Tryptase (TPS) BioAssay™ ELISA Kit (Human) 48Tests　359440

特徴

• The Tryptase (TPS) BioAssay™ ELISA Kit is a quantitative sandwich assay for the detection of TPS in human serum, plasma and other biological fluids. Cell lysates and tissue homogenates, though not tested, may potentially be used as samples.
• Detection Range:0.156-10ng/ml
• Sensitivity:<0.094ng/ml
• Kit Components:*359440A: Microtiter Strips, 1x48 wells or 1x96 wells (8x12 wells)
• *359440B: Standard, 2x1 vial359440C: Sample/Standard Dilution Buffer, 1x10ml or 1x20ml359440D: Antibody (Biotin) (Concentrated), 1x60ul or 1x120ul359440E: Antibody Dilution Buffer, 1x5ml or 1x10ml359440F: Streptavidin (HRP) (SABC), 1x60ul or 1x120ul359440G: SABC Dilution Buffer, 1x5ml or 1x10ml359440H: TMB Substrate, 1x5ml or 1x10ml359440J: Stop Solution, 1x5ml or 1x10ml359440K: Wash Buffer, 25X, 1x15ml or 1x30ml
• Storage and Stability:Store unopened *359440A at 4ºC; store at -20ºC once opened. Store unopened *359440B at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
• Assay Summary:1. Wash plate 2 times before adding standards and samples to wells!2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37°C. Aspirate and wash 2 times
• 3. Add 100ul Antibody (Biotin) working solution to each well and incubate for 60 minutes at 37°C. 4. Aspirate and wash 3 times
• 5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times
• 7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately
• 9. Calculate results.