85-4603-10　［取扱停止］Semaphorin-7A (SEMA7A) BioAssay™ ELISA Kit (Rat) 48Tests　358777

特徴

• This Semaphorin-7A (SEMA7A) BioAssay™ ELISA Kit (Rat) allows for the in vitro quantitative determination of SEMA7A concentrations in serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity:0.188ng/ml
• Range:0.313-20ng/ml
• Precision:Intra-Assay: CV<8%Inter-Assay: CV<10%
• Test Priciple:Semaphorin 7A (SEMA7A) BioAssay™ ELISA Kit (Rat) utilizes the sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.