85-4601-82　［取扱停止］sCD14 (Soluble Cluster of Differentiation14) BioAssay™ ELISA Kit (Human) 48Tests　358701

特徴

• The sCD14 (Soluble Cluster of Differentiation14) BioAssay™ ELISA Kit is a quantitative sandwich assay for the detection of sCD14 in human serum, plasma, tissue homogenates and other biological fluids
• Range:0.313-20ng/ml
• Sensitivity:0.188ng/ml
• Precision: Intra-Assay CV: <8%Inter-Assay CV: <10%
• Kit Components:*358701A: Microtiter Strips, 1x96 wells (8x12 wells)
• *358701B: Standard, 2x1 vial358701C: Sample/Standard Dilution Buffer, 1x20ml358701D: Antibody (Biotin) (Concentrated), 1x120ul358701E: Antibody Dilution Buffer, 1x10ml358701F: Streptavidin (HRP) (SABC), 1x120ul358701G: SABC Dilution Buffer, 1x10ml358701H: TMB Substrate, 1x10ml358701J: Stop Solution, 1x10ml358701K: Wash Buffer, 25X, 1x30ml
• Storage and Stability:Store unopened *358701A at 4ºC; store at -20ºC once opened. Store unopened *358701B at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
• Assay Summary:1. Wash plate 2 times before adding standards and samples to wells!2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37°C. Aspirate and wash 2 times
• 3. Add 100ul Antibody (Biotin) working solution to each well and incubate for 60 minutes at 37°C. 4. Aspirate and wash 3 times
• 5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times
• 7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately
• 9. Calculate results.