85-4580-62　［取扱停止］Neopterin BioAssay™ ELISA Kit (Human) 96Tests　357575

特徴

• The Neopterin BioAssay™ ELISA Kit (Human) is a quantitative competitive assay for the detection of Neopterin in serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity:0.938ng/ml
• Detection Range:1.563-100ng/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of Neopterin. No significant crossreactivity or interference between Neopterin and analogues was observed.
• Precision:Intra-Assay: CV<8%Inter-Assay: CV<10%
• Test Principle:The microtiter plate provided in this kit has been pre-coated withtarget. During the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve.
• Kit Components:*357575A: Microtiter Strips, 8x12 wells
• *357575B: Standard, 2x1 vial357575C: Sample/Standard Dilution Buffer, 1x20ml357575D: Antibody-Biotin Concentrate, 1x160ul357575E: Antibody Dilution Buffer, 1x10ml357575F: Streptavidin-HRP Conjugate (SABC), 1x120ul357575G: SABC Dilution Buffer, 1x10ml357575H: TMB Substrate, 1x10ml357575J: Stop Solution, 1x10ml357575K: Wash Buffer, 25X, 1x30ml
• Storage and Stability:Store unopened *357575A at 4ºC; store at -20ºC once opened. Store unopened *357575B at 4°C; once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
• Assay Summary:1. Wash plate 2 times before adding standards and samples to wells!2. Add 50ul standard or sample to each well
• 3. Add 50ul Antibody (Biotin) working solution to each well and incubate for 45 minutes at 37°C4. Aspirate and wash 3 times
• 5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times
• 7. Add 90ul TMB substrate. Incubate 10-20 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately
• 9. Calculate results