85-4564-82　IL-35 (Interleukin 35) BioAssay™ ELISA Kit (Human) 96Tests　356731

特徴

• The IL-35 (Interleukin 35) BioAssay™ ELISA Kit is a quantitative sandwich assay for the detection of IL-35 in human serum, plasma and other biological fluids. Cell lysates and tissue homogenates, though not tested, may potentially be used as samples.
• Range:15.625-1000pg/ml
• Sensitivity:<9.375pg/ml
• Precision:Intra-assay CV: <8%Inter-assay CV: <10%
• Assay Principle:The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-35. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to IL-35. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL-35, biotin-conjugated antibody and enzyme-conjugated streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IL-35 in the sample is then determined by comparing the O.D. of the sample to the standard curve.
• Kit Components:*356731A: Microtiter Strips, 1x96 wells (8x12 wells)
• *356731B: Standard, 2x1 vial356731C: Sample/Standard Dilution Buffer, 1x20ml356731D: Antibody (Biotin) (Concentrated), 1x120ul356731E: Antibody Dilution Buffer, 1x10ml356731F: Streptavidin (HRP) (SABC), 1x120ul356731G: SABC Dilution Buffer, 1x10ml356731H: TMB Substrate, 1x10ml356731J: Stop Solution, 1x10ml356731K: Wash Buffer, 25X, 1x30ml
• Storage and Stability:Store unopened *356731A at 4ºC; store at -20ºC once opened. Store unopened *356731B at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
• Assay Summary:1. Wash plate 2 times before adding standards, samples and control (zero) to wells!2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37°C. Aspirate and wash 2 times
• 3. Add 100ul Antibody (Biotin) working solution to each well and incubate for 60 minutes at 37°C4. Aspirate and wash 3 times
• 5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times
• 7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately
• 9. Calculate results