85-4562-67　IL-12 (Interleukin 12) BioAssay™ ELISA Kit (Human) 48Tests　356618

特徴

• IL-12 (Interleukin 12) BioAssay™ ELISA Kit is for the quantitative detection of IL-12 in human serum, plasma and other biological fluids. Cell lysates and tissue homogenates, though not tested, may potentially be used as samples.
• Range:15.625-1000pg/ml
• Sensitivity:<9.375pg/ml
• Precision:Intra-assay CV: <8%Inter-assay CV: <10%
• Assay Principle:This kit was based on sandwich enzyme-linked immune-sorbent assay technology. IL-12 antibody was pre-coated onto 96-well plates. And the biotin conjugated IL-12 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrate was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-10 amount of sample captured on plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-12 can be calculated.
• Kit Components:356618A: Microtiter Strips, 1x96 wells 356618B: Standard, 2x1vial356618C: Sample/Standard Dilution Buffer, 1x20ml356618D: Antibody (Biotin) (Concentrated), 1x120ul356618E: Antibody Dilution Buffer, 1x10ml356618F: Streptavidin (HRP) (SABC), 1x120ul356618G: SABC Dilution Buffer, 1x10ml356618H: TMB Substrate, 1x10ml356618J: Stop Solution, 1x10ml356618K: Wash Buffer, 25X, 1x30ml
• Storage and Stability:Store unopened *356618A at 4ºC. Once opened, store at -20ºC. Store *356618B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Assay Summary:1. Wash plate 2 times before adding standards, samples and control (zero) to wells!2. Add 100ul standard or sample to each well for 90 minutes at 37°C3. Add 100ul Biotin antibody working solution to each well for 60 minutes at 37°C4. Aspirate and wash 3 times5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times7. Add 90ul TMB substrate. Incubate 10-20 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately
• 9. Calculation of results