85-4560-95　［取扱停止］IGF-1 (Insulin-Like Growth Factor 1) BioAssay™ ELISA Kit (Human) 48Tests　356525

特徴

• The IGF-1 (Insulin-Like Growth Factor 1) BioAssay™ ELISA Kit (Human) is a quantitative sandwich assay for the detection of IGF-1 in human serum, plasma and other biological fluids. Cell and tissue homogenates have not been tested but may potentially be used as samples.
• Detection Range:62.5 – 4,000pg/ml
• Sensitivity:<37.5pg/ml
• Precision:Intra-assay CV: <8%Inter-assay CV: <10%
• Assay Principle:The microtiter plate provided in this kit has been pre-coated with an antibody specific to IGF-1. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to IGF-1. Streptavidin conjugated to horseradish peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IGF-1, biotin-conjugated antibody and enzyme-conjugated streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IGF-1 in the sample is then determined by comparing the O.D. of the sample to the standard curve.
• Kit Components:356525A: Microtiter Strips, 8x12 wells
• 356525B: Standard, 2 vials356525C: Sample/Standard Dilution Buffer, 1x20ml356525D: Antibody-Biotin (Concentrated), 1x120ul356525E: Antibody Dilution Buffer, 1x10ml356525F: Streptavidin-HRP Conjugate (SABC), 1x120ul356525G: SABC Dilution Buffer, 1x10ml356525H: TMB Substrate, 1x10ml356525J: Stop Solution, 1x10ml356525K: Wash Buffer (25X), 1x30ml
• Storage and Stability:Store unopened 356525A at 4ºC; store at -20ºC once opened. Store unopened 356525B at 4°C; once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store 356525F and 356525H in the dark at 4°C. Store all other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
• Assay Summary:1. Wash plate 2 times before adding standards, samples and control (zero) to wells
• 2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37°C 3. Aspirate the liquid and wash plate 3 times
• 3. Add 100ul Antibody-Biotin working solution to each well and incubate for 60 minutes at 37°C4. Aspirate the liquid and wash plate 3 times
• 5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate the liquid and wash plate 5 times
• 7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately
• 9. Calculate results.