85-4560-11　［取扱停止］IFN-beta (Interferon Beta) BioAssay™ ELISA Kit (Rat) 48Tests　356481

特徴

• The IFN-beta (Interferon Beta) BioAssay™ ELISA Kit (Rat) is used for the quantitative detection of IFN-beta in serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity:9.375pg/ml
• Range:15.625-1000pg/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of rat IFN-beta. No significant cross-reactivity or interference between IFN-beta and analogues was observed.
• Precision:Intra-Assay: CV<8%Inter-Assay: CV<10%
• Test Principle:This BioAssay™ kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IFN-beta antibody was pre-coated onto 96-well plates. The biotin conjugated anti-IFN-beta antibodywas used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates wereused to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IFN-beta amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IFN-beta can be calculated.
• Kit Components:Micro ELISA Plate(Dismountable), 8×12Lyophilized Standard, 2x vialSample / Standard dilution buffer, 1x20mlBiotin-detection antibody (Concentrated), 1x120ulAntibody dilution buffer, 1x10mlHRP-Streptavidin Conjugate(SABC), 1x120ulSABC dilution buffer, 1x10mlTMB substrate, 1x10mlStop solution, 1x10mlWash buffer (25X), 1x30mlPlate Sealer, 1x5pieces
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.