85-4559-91　［取扱停止］IFN-alpha (Interferon Alpha) BioAssay™ ELISA Kit (Monkey) 96Tests　356471

特徴

• The Interferon alpha (IFN-alpha) BioAssay™ ELISA Kit (monkey) is a quantitative competitive assay for the detection of IFN-alpha in monkey serum, plasma, tissue homogenates and other biological fluids.
• Detection Range:15.625-1000pg/ml
• Sensitivity:<9.375pgl/ml
• Precision: Intra-Assay CV: <8%Inter-Assay CV: <10%
• Assay Principle:The microtiter plate provided in this kit has been pre-coated with an antibody specific to IFN-alpha. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to IFN-alpha. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IFN-alpha, Biotin-conjugated antibody and enzyme-conjugated Streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of IFN-alpha in the sample is then determined by comparing the O.D. of the sample to the standard curve.
• Kit Components:*356471 A: Microtiter Strips, 8x12 wells
• *356471B: Standard, 2 vials356471C: Sample/Standard Dilution Buffer, 1x20ml356471D: Antibody (Biotin) Concentrate, 1x120ul356471E: Antibody Dilution Buffer, 1x10ml356471F: Streptavidin (HRP) Conjugate (SABC), 1x120ul356471G: SABC Dilution Buffer, 1x10ml356471H: TMB Substrate, 1x10ml356471J: Stop Solution, 1x10ml356471K: Wash Buffer, 25X, 1x30ml
• Storage and Stability:Store unopened *356471A at 4ºC; store at -20ºC once opened. Store unopened *356471B at 4°C; once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
• Assay Summary:1. Wash plate 2 times before adding standards, samples and control (zero) to wells
• 2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37ºC
• 3. Aspirate and wash 2 times
• 4. Add 100ul Antibody-Biotin working solution to each well and incubate for 60 minutes at 37°C5. Aspirate and wash 3 times
• 6. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C7. Aspirate and wash 5 times
• 8. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C9. Add 50ul Stop Solution. Read at 450nm immediately
• 10. Calculate results.