85-4542-53　FA/VB9 (Folic Acid/Vitamin B9) BioAssay™ ELISA Kit (Universal) 48Tests　355547

特徴

• The FA/VB9 (Folic Acid/Vitamin B9) BioAssay™ ELISA Kit is a quantitative competitive assay for the detection of Vitamin B12 in serum, plasma, tissue homogenates and other biological fluids.
• Range:12.5-800ng/ml
• Sensitivity:<7.5ng/ml
• Precision:Intra-assay CV: <8%Inter-assay CV: <10%
• Assay Principle:This ELISA kit employs the quantitative competitive enzyme-linked immunoassay technique, utilizing a microtiter plate provided in this kit has been pre-coated with an antibody specific to FA/VB9. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to FA/VB9. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FA/VB9, Biotin-conjugated antibody and enzyme-conjugated Streptavidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FA/VB9 in the sample is then determined by an inverse correlation between FA/VB9 in the samples and the assay signal intensity.
• Kit Components:*355547A: Microtiter Strips, 1x96 wells (8x12 wells)
• *355547B: Standard, 2x1 vials355547C: Sample/Standard Dilution Buffer, 1x20ml355547D: Antibody (Biotin) (Concentrated), 1x60ul355547E: Antibody Dilution Buffer, 1x10ml355547F: Streptavidin (HRP) (SABC), 1x120ul355547G: SABC Dilution Buffer, 1x10ml355547H: TMB Substrate, 1x10ml355547J: Stop Solution, 1x10ml355547K: Wash Buffer, 25X, 1x30ml
• Storage and Stability:Store unopened *355547A at 4ºC; store at -20ºC once opened. Store unopened *355547B at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
• Assay Summary:1. Wash plate 2 times before adding standards, samples and control (zero) to wells!2. Add 50ul standard or sample to each well. Immediately add 50ul (Biotin) working solution to each well
• 3. Incubate for 45 minutes at 37°C4. Aspirate and wash 3 times
• 5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times
• 7. Add 90ul TMB substrate. Incubate 10-20 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately
• 9. Calculate results.