85-4512-88　［取扱停止］Anti-AChR (Anti-Acetylcholine Receptor Antibody) BioAssay™ ELISA Kit (Human) 96Tests　353958

特徴

• The Anti-AChR (Anti-Acetylcholine Receptor Antibody) BioAssay™ ELISA Kit (Human) is a quantitative indirect assay for the detection of Anti-AChR antibody in human serum, plasma and other biological fluids.
• Detection Range:1.563–100ng/ml
• Sensitivity:<0.938ng/ml
• Precision:Intra-Assay CV: <8%Inter-Assay CV: <10%
• Assay Principle:The microtiter plate provided in this kit has been pre-coated with Acetylcholine Receptor. The standards and test samples are added into the respective wells and the plate is incubated and washed. Biotinylated goat anti-human IgG is then added to each well, followed by incubation and washing steps. HRP-Streptavidin is added and unbound materials are removed by washing. HRP-catalyzed reaction of the TMB substrate results in a blue colored product which changes into yellow upon addition of the stop solution. The absorbance measured at 450nm is directly proportional to the concentration of Anti-AChR antibody in the samples and standards. The concentration of Anti-AChR antibody in the samples is determined by comparing the absorbance of the samples to the standard curve.
• Kit Components:353958A: Microtiter Strips, 8x12 wells
• 353958B: Standard, 2 vials353958C: Sample/Standard Dilution Buffer, 1x20ml353958D: Antibody-Biotin Concentrate, 1x160ul353958E: Antibody Dilution Buffer, 1x10ml353958F: Streptavidin-HRP Conjugate (SABC), 1x120ul353958G: SABC Dilution Buffer, 1x10ml353958H: TMB Substrate, 1x10ml353958J: Stop Solution, 1x10ml353958K: Wash Buffer (25X), 1x30ml
• Storage and Stability:Store unopened 353958A at 4°C; store at -20°C once opened. Store unopened 353958B at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store 353958F and 353958H protected from light at 4°C. Store the rest of the components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
• Assay Summary:1. Wash plate 2 times before adding standards, samples and control (zero) to wells
• 2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37°C 3. Aspirate the liquid and wash plate 2 times
• 3. Add 100ul Antibody-Biotin working solution to each well and incubate for 60 minutes at 37°C4. Aspirate the liquid and wash plate 3 times
• 5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate the liquid and wash plate 5 times
• 7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately
• 9. Calculate results.