85-4511-10　［取扱停止］Melanocyte-Stimulating Hormone Alpha (Alpha-MSH) BioAssay™ ELISA Kit (Mouse) 48Tests　353854

特徴

• The Melanocyte-Stimulating Hormone Alpha (Alpha-MSH) BioAssay™ ELISA Kit (Mouse) is a quantitative sandwich assay for the detection of Alpha-MSH in mouse serum, plasma, tissue homogenates and other biological fluids.
• Detection Range:15.625-1000pg/ml
• Sensitivity:<9.375pg/ml
• Precision:Intra-Assay CV: <8%Inter-Assay CV: <10%
• Assay Principle:The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-MSH. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to Alpha-MSH. Streptavidin conjugated to horseradish peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-MSH, biotin-conjugated antibody and enzyme-conjugated streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-MSH in the sample is then determined by comparing the O.D. of the sample to the standard curve.
• Kit Components:353854A: Microtiter Strips, 8x12 wells
• 353854B: Standard, 2 vials353854C: Sample/Standard Dilution Buffer, 1x20ml353854D: Antibody-Biotin (Concentrated), 1x120ul353854E: Antibody Dilution Buffer, 1x10ml353854F: Streptavidin-HRP Conjugate (SABC), 1x120ul353854G: SABC Dilution Buffer, 1x10ml353854H: TMB Substrate, 1x10ml353854J: Stop Solution, 1x10ml353854K: Wash Buffer (25X), 1x30ml
• Storage and Stability:Store unopened 353854A at 4ºC; store at -20ºC once opened. Store unopened 353854B at 4°C; once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store 353854F and 353854H protected from light at 4°C. Store all other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
• Assay Summary:1. Wash plate 2 times before adding standards, samples and control (zero) to wells
• 2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37°C 3. Aspirate the liquid and wash plate 3 times
• 3. Add 100ul Antibody-Biotin working solution to each well and incubate for 60 minutes at 37°C4. Aspirate the liquid and wash plate 3 times
• 5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate the liquid and wash plate 5 times
• 7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately
• 9. Calculate results.