85-4505-33　4-Hydroxynonenal (4-HNE) BioAssay™ ELISA Kit (Human) 96Tests　353545

特徴

• The 4-Hydroxynonenal (4-HNE) BioAssay™ ELISA Kit is a quantitative competitive assay for the detection of 4-HNE in human serum, plasma and other biological fluids. Cell lysates and tissue homogenates, though not tested, may potentially be used as samples.
• Detection Range:31.25-2000pg/ml
• Sensitivity:18.75pg/ml
• Precision: Intra-Assay CV: <8%Inter-Assay CV: <10%
• Assay Principle:This ELISA kit employs the quantitative competitive enzyme-linked immunoassay technique, utilizing a microtiter plate provided in this kit has been pre-coated with 4-HNE. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to 4-HNE. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 4-HNE, Biotin-conjugated antibody and enzyme-conjugated Streptavidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 4-HNE in the sample is then determined by comparing the O.D. of the sample to the standard curve.
• Kit Components:*353545A: Microtiter Strips, 1x48 wells or 1x96 wells *353545B: Standard, 1x1 vial or 2x1 vials353545C: Sample/Standard Dilution Buffer, 1x10ml or 1x20ml353545D: Antibody (Biotin) (Concentrated), 1x30ul or 1x60ul353545E: Antibody Dilution Buffer, 1x5ml or 1x10ml353545F: Streptavidin (HRP) (SABC), 1x60ul or 1x120ul353545G: SABC Dilution Buffer, 1x5ml or 1x10ml353545H: TMB Substrate, 1x5ml or 1x10ml353545J: Stop Solution, 1x5ml or 1x10ml353545K: Wash Buffer, 25X, 1x15ml or 1x30ml
• Storage and Stability:Store unopened *353545A at 4ºC; store at -20ºC once opened. Store unopened *353545B at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
• Assay Summary:1. Wash plate 2 times before adding standards and samples to wells
• 2. Add 50ul standard or sample to each well
• 3. Immediately add 50ul Antibody-Biotin working solution to each well and incubate for 45 minutes at 37°C4. Aspirate and wash 3 times
• 5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times
• 7. Add 90ul TMB substrate. Incubate 10-20 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately
• 9. Calculate results.