Sample Type:Yeast and other expression system, tissue
Applications:Levels of MAT1A/1B, MAT2A gene expressions. Extent of turning methionine into active methionine, i.e., SAM; related to methylation capability.
Specificity:Methionine Adenosyltransferase (MAT)
Test Principle:This BioAssay™ kit is designed to calculate MAT activity through measuring the concentration of the main product SAM. The chemical reaction and the competition cELISA are carried out simultaneously, so that the SAM synthesized from the chemical reaction competes with antigens coated on the micro-titer plate for the horseradish peroxidase conjugated anti-SAM antibody the minute it is generated. The SAM quantified based on the amount of the HRP-anti-SAM antibodies that was made not to bind to the micro-titer plate.
Kit Components:Wash Solution, 20x, 1x20mlAssay Plate, 12x8wellsHRP-antibody Diluent, 1x9mlHRP-antibody, 1x15ulMAT Substrate, 1x1mlSample Diluent, 1x6mlMAT Positive Control, 1x40ulHRP Substrate, 1x11mlStop Solution, 1x6mlMicroplate Sealers, 2S0 SAM Standard, 1x200ulS1 SAM Standard, 15nM, 1x200ulS2 SAM Standard, 30nM, 1x200ulS3 SAM Standard, 120nM, 1x200ulS4 SAM Standard, 240nM, 1x200ulS5 SAM Standard, 480nM, 1x200ulS6 SAM Standard, 960nM, 1x200ulQC SAM Quality Control, 160-240nM, 1x200ul
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
