Tetranectin, also called TNA, is a protein that in humans is encoded by the CLEC3B gene. It is mapped to 3p21.31. Tetranectin, a tetrameric protein isolated from human plasma, has 4 identical and noncovalently bound polypeptide chains, each of 181aa residues. It has a specific binding affinity for sulfated polysaccharides and the kringle 4 of plasminogen. The plasma concentration of tetranectin is reduced in patients with various malignancies. Tetranectin is a plasminogen-binding protein that is induced during the mineralization phase of osteogenesis. Thus, tetranectin is a candidate gene for human disorders affecting bone and connective tissue.
Sample Type:Cell culture supernates, serum and tissue homogenates
Intended Use:For quantitative detection of human Tetranectin in cell culture supernates, serum and tissue Homogenates.
Sensitivity:<10pg/ml
Range:312-20,000pg/ml
Specificity:Natural and recombinant human Tetranectin
Test Principle:This human Tetranectin BioAssay™ ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Tetranectin has been precoated onto 96-well plates. Standards(NSO, E22-V202) and test samples are added to the wells; a biotinylated detection polyclonal antibody from goat specific for Tetranectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Tetranectin amount of sample captured in plate.
Kit Components:96-well plate precoated with human Tetranectin antibody, 1ST0000-20, Lyophilized recombinant human Tetranectin standard, 20ng/tube×2Biotinylated human Tetranectin antibody, 130ul (dilution 1:100)Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100)Sample diluent buffer, 30mlAntibody diluent buffer, 12mlABC diluent buffer, 12mlTMB color developing agent, 10mlTMB stop solution, 10ml
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
