Lumican, also known as LUM, is a protein which in humans is encoded by the LUM gene. This gene is a member of the small interstitial proteoglycan gene(SIPG) family, and it is a keratan sulfate proteoglycan which presents large quantities in the corneal stroma and in interstitial collagenous matrices of the heart, aorta, skeletal muscle, skin, and intervertebral discs. Lumican is mapped to 12q21.33, it interacts with collagen and limits growth of fibrils in diameter. In the cornea, Lumican not only interacts with collagen molecules to limit fibril growth, but also plays a critical role in the regular spacing of fibrils and acquisition of corneal transparency by virtue of its keratan sulfate-containing glycosaminoglycan side chains LDC.
Sample Type:Cell culture supernates, serum and plasma (heparin)
Intended Use:For quantitative detection of rat Lumican in cell culture supernates, serum and plasma(heparin).
Sensitivity:<10pg/ml
Range:156-10,000pg/ml
Specificity:Natural and recombinant rat Lumican
Test Principle:This rat Lumican BioAssay™ ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Lumican has been precoated onto 96-well plates. Standards(NSO, Q19-N338) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Lumican is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Lumican amount of sample captured in plate.
Kit Components:96-well plate precoated with rat Lumican antibody, 1Lyophilized recombinant rat Lumican standard, 10ng/tube×2Biotinylated rat Lumican antibody, 130ul (dilution 1:100)Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100)Sample diluent buffer, 30mlAntibody diluent buffer, 12mlABC diluent buffer, 12mlTMB color developing agent, 10mlTMB stop solution, 10ml
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
