Kit Components:1. Normal goat serum blocking reagent: 10x, 10ml, for the block of tissue sections. 2. Biotinylated Secondary Antibody (Goat Anti-rabbit IgG): 100~200x, 1ml (2mg/ml). Affinity purified antibody, labeled with “long-arm” biotin (Biotinamidohexanoic acid N-hydroxysuccinimide ester,
3. SABC-POD (HRP conjugated streptavidin): 100~200x, 1ml (2mg/ml). 4. Three drop bottles (For dilution use).
Applications:Suitable for use in Immunohistochemistry (paraffin), Immunohistochemistry (frozen), Immunocytochemistry. Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1:50-200Immunohistochemistry (frozen): 1:50-200Immunocytochemistry: 1:50-200Optimal dilutions to be determined by the researcher.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
