SABC (StreptAvidin-Biotin Complex) is specially designed for displaying the distribution of antigens in tissues and cells in immunochemistry and other immunodetection analyses. This kit has high sensitivity because each complex it generates has a large number of FITC or peroxidase, and streptavidin molecules. Compared to the traditional immunodetection using free FITC dyes, the SABC-FITC system greatly enhances the sensitivity and suppresses the background. FITC excitation is at 490~495nm, emission at 520~530nm.
3. SABC-FITC+HRP (FITC and HRP conjugated streptavidin): 100x, 0.5ml (2mg/ml). 4. Three drop bottles (For dilution use).
Applications:Suitable for use in Immunohistochemistry (paraffin), Immunohistochemistry (frozen), Immunocytochemistry. Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1:50-200Immunohistochemistry (frozen): 1:50-200Immunocytochemistry: 1:50-200Optimal dilutions to be determined by the researcher.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
