SABC (StreptAvidin-Biotin Complex) is specially designed for displaying the distribution of antigens in tissues and cells in immunochemistry and other immunodetection analyses. This kit has high sensitivity because each complex it generates has a large number of Cy3 and streptavidin molecules. Compared to the traditional immunodetection using free Cy3 dyes, the SABC-Cy3 system greatly enhances the sensitivity and suppresses the background. Cy3 is activated at 554nm, fluorescing at 568~574nm with bright red.
Kit Components:1. Normal rabbit serum blocking reagent: 10x, 5ml, for the block of tissue sections. 2. Biotinylated Secondary Antibody (Rabbit Anti-goat IgG): 100x, 0.5ml (2mg/ml). Affinity purified antibody, labeled with “long-arm” biotin (Biotinamidohexanoic acid N-hydroxysuccinimide ester,
3. SABC-Cy3 (Cy3 conjugated streptavidin): 100x, 0.5ml (1mg/ml). 4. Three drop bottles (For dilution use).
Applications:Suitable for use in Immunohistochemistry (paraffin), Immunohistochemistry (frozen), Immunocytochemistry. Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1:50-200Immunohistochemistry (frozen): 1:50-200Immunocytochemistry: 1:50-200Optimal dilutions to be determined by the researcher.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
