SIGLEC-1, also known as Sialoadhesin or CD169, is a cell adhesion molecule found on the surface of certain cells of the immune system called macrophages. This gene is mapped to 20p13. It belongs to the immunoglobulin superfamily(IgSF). Since sialoadhesin binds sialic acids with its N-terminal IgV-domain, it is also a member of the SIGLEC family. The localization and expression of SIGLEC-1 in humans has altered coincident with the evolutionary loss of Neu5Gc. SIGLEC-1-positive macrophages have a dual physiologic function. They act as innate 'flypaper' by preventing the systemic spread of lymph-borne pathogens and as critical gatekeepers at the lymph-tissue interface that facilitate the recognition of particulate antigens by B cells and initiate humoral immune responses.
Sample Type:Cell culture supernates, serum and plasma (heparin, EDTA)
Intended Use:For quantitative detection of human CD169 in cell culture supernates, serum and plasma (heparin, EDTA).
Sensitivity:<10pg/ml
Range:156-10,000pg/ml
Specificity:Natural and recombinant human CD169
Test Principle:This human CD169 BioAssay™ ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD169 has been precoated onto 96-well plates. Standards(NSO, S20-Q1641) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD169 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD169 amount of sample captured in plate.
Kit Components:96-well plate precoated with human CD169 antibody, 1Lyophilized recombinant human CD169 standard, 10ng/tube×2Biotinylated human CD169 antibody, 130ul (dilution 1:100)Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100)Sample diluent buffer, 30mlAntibody diluent buffer, 12mlABC diluent buffer, 12mlTMB color developing agent, 10mlTMB stop solution, 10ml
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
