Intended Use:The Malachite Green Phosphate Detection Kit is a convenient and sensitive, single-step free-phosphate determination kit that can be used for measuring phosphate released during enzymatic phosphatase assays.
Range:Linear range of 50-1600pmol of phosphate.
Specificity:The Malachite Green Phosphate Detection Kit detects only free-phosphate in sample.
Test Principle:The Malachite Green Phosphate Detection BioAssay™ Kit is designed for the in vitro measurement of phosphatase activity. Protein phosphatase enzymatic activity results in the release into solution of free inorganic phosphate from protein-phosphatase substrates. This free inorganic phosphate then forms a green molybdophosphoric acid complex. Formation of this complex is monitored and quantified by measuring the absorbance at 630nm. This absorbance is proportional to free-inorganic phosphate in solution and correlates with the activity of phosphatase (1,2).
Kit Components:Malachite Green Reagent, 2x28mlPhosphate Standard (1mM), 1x0.5ml
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
