85-4498-41　Tissue Inhibitors of Metalloproteinase 4 (TIMP-4) BioAssay™ ELISA Kit (Human) 96Tests　351709

特徴

• This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling.
• Sample Type:Serum, plasma, tissue homogenates, human milk
• Intended Use:For the quantitative determination of human tissue inhibitors of metalloproteinase 4 (TIMP-4) concentrations in serum, plasma, tissue homogenates.
• Sensitivity:0.005ng/ml
• Range:0.313-20ng/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of human TIMP-4. No significant cross-reactivity or interference between human TIMP-4 and analogues was observed.
• Test Principle:This BioAssay™ employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TIMP-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TIMP-4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIMP-4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIMP-4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate (12x8 coated microwells), 1x96 wellsStandard (Freeze dried), 2x vialsBiotin-antibody (100X), 1x120ulHRP-avidin (100X), 1x120ulBiotin-antibody Diluent, 1x15mlHRP-avidin Diluent, 1x15mlSample Diluent, 1x50mlWash Buffer (25X), 1x20mlTMB Substrate, 1x10mlStop Solution, 1x10mlAdhesive Strip (For 96 wells), 4x stripsInstruction manual
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.