85-4498-33　［取扱停止］Thyroglobulin BioAssay™ ELISA Kit (Human) (TG, AITD3, TGN) 24Tests　351706

特徴

• Thyroglobulin (Tg) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis.
• Sample Type:Serum, plasma, tissue homogenates
• Intended Use:For the quantitative determination of human Thyroglobulin(TG) concentrations in serum, plasma, tissue homogenates.
• Sensitivity:1.56ng/ml
• Range:6.25-400ng/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of human TG. No significant cross-reactivity or interference between human TG and analogues was observed.
• Test Principle:This BioAssay™ employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TG bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate (12x8 coated microwells), 1x96 wellsStandard (Freeze dried), 2x vialsBiotin-antibody (100X), 1x120ulHRP-avidin (100X), 1x120ulBiotin-antibody Diluent, 1x15mlHRP-avidin Diluent, 1x15mlSample Diluent, 1x50mlWash Buffer (25X), 1x20mlTMB Substrate, 1x10mlStop Solution, 1x10mlAdhesive Strip (For 96 wells), 4x stripsInstruction manual
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.