85-4497-91　Rotavirus (RV) antigen (Ag) BioAssay™ ELISA Kit (Sheep) 48Tests　351693

特徴

• Sample Type:Feces
• Intended Use:For the qualitative determination of sheep rotavirus (RV) antigen (Ag) concentrations in feces.
• Test Principle:This BioAssay™ employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rotavirus antigen. Samples are pipetted into the wells with antibody conjugated Horseradish Peroxidase(HRP). Any Rotavirus antigen present in samples will bind to the pre-coated specific antibody. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sheep rotavirus (RV) antigen (Ag) bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Specificity:This assay has high sensitivity and excellent specificity for detection of sheep rotavirus (RV) antigen (Ag). No significant cross-reactivity or interference between sheep rotavirus (RV) antigen (Ag) and analogues was observed.
• Kit Components:Assay plate, 1x96 wellsNegative Control, 1x800ulPositive Control, 1x800ulHRP-conjugate, 1x6mlWash Buffer, 1x6mlSubstrate A, 1x5mlSubstrate B, 1x5mlStop Solution, 1x5mlAdhesive Strip (For 96 wells), 4x stripsInstruction manual
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.