85-4497-59　Plasminogen (PLG) BioAssay™ ELISA Kit (Mouse) 96Tests　351682

特徴

• Plasminogen (PLG), BioAssay™ ELISA Kit (Mouse) is a competitive ELISA intended for the quantitative determination of PLG concentration in mouse serum, plasma and tissue homogenates.
• Sensitivity:<15.6ng/ml
• Range:62.5-4000ng/ml
• Precision:Intra-assay CV: <8%Inter-assay CV: <10%
• Principle of the Assay:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PLG. Standards or samples are added to the appropriate microtiter plate wells with HRP-conjugated anti-PLG antibody. A competitive inhibition reaction is launched between pre-coated PLG and PLG in the standards or samples. A substrate solution is added to the wells; the color development is stopped and the intensity of the color is measured. The intensity of the color is inversely proportional to the amount of PLG in the samples.
• Kit Components:227665A: Microtiter Strips (12 x 8 microwells). Pre-coated with PLG; ready to use. 227665B: Standard (Lyophilized), 2x1 vials227665C: HRP Conjugate (100X Concentrate), 1x60ul227665D: HRP Conjugate Diluent, 1x10ml227665E: Sample Diluent 2x20ml227665F: Wash Buffer (25X Concentrate), 1x20ml227665G: TMB Substrate, 1x10ml227665H: Stop Solution, 1x10ml227665J: Wash Buffer (25X Concentrate), 1x20ml
• Storage and Stability:Unopened kit: Store at 4°C. Unused kit is stable for 6 months after receipt. Opened Kit: Once opened, the following kit components are stable for up to 1 month at 4°C providing this is within the expiration date of the kit: HRP conjugate diluent, Sample diluent, Wash buffer, TMB substrate and Stop solution. The coated plate, standard and HRP conjugate may be stored at 4°C for up to one month; store the standard and HRP conjugate at -20°C if the kit will not be used within one month. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
• Assay Summary:1. Prepare reagents, working standards and samples as instructed
• 2. Set a Blank well without any solution3. Add 50ul Standard or sample to each well
• 4. Add 50ul HRP Conjugate to each well (do not add any to the blank well)
• 5. Incubate for 60 minutes at 37°C
• 6. Aspirate and wash 5 times
• 7. Add 90ul TMB substrate to each well8. Incubate for 20 minutes at 37°C. Protect from light12. Add 50ul Stop Solution to each well. Read at 450nm within 5 minutes